AAT Bioquest公司熒光探針Rhod-4™使用說明

蘇州螞蟻淘生物科技有限公司是AATBIO的中國大陸區授權代理商

美國(guo)AAT Bioquest Inc.(前身是(shi)ABD Bioquest，Inc.)是一家為從事生命科學研究、診斷研發及藥物開發的科學家研發、生產和銷售生物分析研究試劑和試劑盒的公司。公司致力于光譜學檢測領域，包括吸收(顏色)、熒(ying)光和發光技術。AAT Bioquest的產品(pin)幫助*的科學(xue)家和生(sheng)物(wu)醫藥(yao)研究者(zhe)更好的了解生(sheng)物(wu)化學(xue)、免疫學(xue)、細胞(bao)生(sheng)物(wu)學(xue)和分子生(sheng)物(wu)學(xue)等(deng)領域。AAT Bioquest會不(bu)斷(duan)研發(fa)新產品，快速地豐富各個領域的產品。

1）提供反應(ying)探(tan)針和發光(guang)探(tan)針，生(sheng)物(wu)素和端粒酶能夠應(ying)用于標記(ji)藥物(wu)小分(fen)子和生(sheng)物(wu)聚合物(wu)，如蛋(dan)白、核酸以及其他碳水(shui)化合物(wu)；

2）研究并生產熒光(guang)和(he)發光(guang)探針用(yong)于(yu)檢測(ce)蛋白，核酸和(he)活細胞；

3）不斷推出新型的熒(ying)光(guang)和發光(guang)探針(zhen)用于(yu)檢測(ce)多(duo)種酶，特別(bie)是檢測(ce)水解酶和氧化還原(yuan)酶類；

4）致力于(yu)開發用于(yu)信號轉導(dao)研究的(de)試(shi)劑；

5）提供生理(li)和神(shen)經探(tan)針，特別(bie)是鈣離(li)子指示劑和膜電位(wei)探(tan)針。

AAT Bioquest公司熒光探針Rhod-4™使用說明

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge

Calculators
Common stock solution preparation 
 

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.