AAT Bioquest公司熒光探針Fluo-8®使用說明

 蘇州螞蟻淘生物科技有限公司是AATBIO的中國大陸區授權代理商

產品介紹：       

         染料DiI, DiO, DiD 和 DiR是(shi)一類親(qin)脂(zhi)性(xing)熒(ying)光(guang)染料家族，用(yong)于標記細胞膜(mo)(mo)和疏水性(xing)組織(zhi)。這(zhe)是(shi)一類環境敏感型(xing)熒(ying)光(guang)染料，當(dang)它們與膜(mo)(mo)結(jie)(jie)合或者與親(qin)脂(zhi)性(xing)生物(wu)分(fen)子(例如蛋白質，雖(sui)然在水中其(qi)熒(ying)光(guang)強(qiang)度很弱)結(jie)(jie)合時(shi)，其(qi)熒(ying)光(guang)強(qiang)度顯著(zhu)增強(qiang)。它們具有(you)很高的(de)淬(cui)滅(mie)系數，偏光(guang)依賴性(xing)和很短的(de)激發壽命。一旦應用(yong)于細胞中，這(zhe)種(zhong)染料會在細胞內質膜(mo)(mo)中逐(zhu)步擴散，導(dao)致(zhi)在其(qi)*濃度條(tiao)件下(xia)，將整個細胞染色(se)。

        它們不同的(de)熒(ying)(ying)光顏色(se)：DiI（橙色(se)熒(ying)(ying)光）、DiO（綠色(se)熒(ying)(ying)光）、DiD（紅(hong)色(se)熒(ying)(ying)光）、DiR（深紅(hong)色(se)熒(ying)(ying)光），為(wei)活細(xi)胞多色(se)彩熒(ying)(ying)光成(cheng)像分析和(he)流式細(xi)胞術提供了一(yi)種便捷(jie)的(de)工具(ju)。DiO和(he)DiI可以分別與標準的(de)FITC和(he)TRITC濾(lv)光器一(yi)同使用(yong)，其中，DiO可以被633 nm He-Ne激光激發(fa)，并且具(ju)有(you)比DiI更長的(de)激發(fa)和(he)發(fa)射(she)光波(bo)長，為(wei)標記細(xi)胞和(he)組織的(de)那些本身就(jiu)具(ju)有(you)本底(di)熒(ying)(ying)光的(de)染料提供了非常的(de)替代品。

        DiR在活體(ti)成(cheng)像(xiang)或者示蹤中非常有用，因為它們所發射的(de)紅(hong)外光可(ke)以(yi)高(gao)效(xiao)地穿過細胞和組織，并且在紅(hong)外光范圍內，其本底熒光水平很(hen)低(di)。

技術資料：

      1.Ex(nm)：748

      2.Em(nm)：780

      3.分子量：

      4.溶劑：DMSO

AAT Bioquest公司熒光探針Fluo-8®使用說明

 Fluo-8® AM is a novel green fluorescent indicator that utilizes a fluorescein core to monitor Ca2+ concentration and flux in cells. It has several key advantages over earlier generations of indicators like Fluo-3 AM and Fluo-4 AM:

• Enhanced Intensity → 2x brighter than Fluo-4 AM
• Better & Faster Loading → Room temperature instead of 37°C
• More Robust Results → Probe effective in a wide range of temperatures
• Variety of K_d → Many options for calcium binding dissociation constant

Fluo-8® AM Features

Fluo-8® AM is a novel fluorescent green calcium indicator that builds upon the fluorescein core while rectifying the issues that plagued earlier indicators like Fluo-3 AM and Fluo-4 AM. In particular, it has several key advantages:

• Enhanced Intensity - Tests have shown Fluo-8® AM is twice as bright as Fluo-4 AM and up to four times brighter than Fluo-3 AM
• Better Loading - Fluo-8® AM can be loaded at room temperature, unlike the 37°C incubation required by Fluo-4 AM
• Faster Loading - Room temperature loading means no need to warm up and cool down cells; finish loading in as little as 20 minutes
• Robust - Fluo-8® is less temperature dependent than other probes, leading to more consistent results
• Versatile K_d - Fluo-8® AM is available with a variety of Ca2+ binding dissociation constants, see Table 1 for details

The most brilliant achievement of Fluo-8® AM is that it improves upon the shortcomings of Fluo-3 AM and Fluo-4 AM without touching the excitation and emission. Like Fluo-3 AM and Fluo-4 AM, Fluo-8® AM will excite at ~490 nm and emit at ~520 nm. This allows for a seamless transition for those who are familiar with Fluo-3 AM and Fluo-4 AM but wish to upgrade to Fluo-8® AM. 

As with its predecessors, Fluo-8® AM is completely compatible with use in flow cytometry, fluorescence microscopy, fluorescence spectroscopy and the fluorescence microplate platform. To read more about how Fluo-8® AM has revolutionized calcium imaging, or to see additional Fluo-8® product applications, please click here. 

Table 1. Ca2+ Binding Dissociation Constants