Lifetech細胞計數儀*

Lifetech細胞計數儀*

2018年1月(yue)份螞蟻淘新到一(yi)批Thermofisher/AMQAF1000/Countess II FL細胞計數儀現貨

歡迎大家仔細訂購

4000-520-616

Thermofisher/AMQAF1000/Countess II FL細胞計數(shu)儀現貨

產品介(jie)紹：

全(quan)新(xin)的Countess® II FL全(quan)自(zi)動(dong)細(xi)胞(bao)計數(shu)(shu)儀是一(yi)款配備了*進(jin)的光(guang)(guang)學(xue)和圖像分析(xi)軟件，可以(yi)(yi)快速對懸液之(zhi)中的細(xi)胞(bao)進(jin)行(xing)評估的臺式細(xi)胞(bao)分析(xi)平臺。現如今(jin)，隨著(zhu)其具(ju)備了熒(ying)光(guang)(guang)應用(yong)能力——明場(chang)和兩路熒(ying)光(guang)(guang)光(guang)(guang)學(xue)通道，研究人員(yuan)可以(yi)(yi)用(yong)之(zhi)進(jin)行(xing)細(xi)胞(bao)計數(shu)(shu)、監測熒(ying)光(guang)(guang)蛋白(bai)表(biao)達以(yi)(yi)及測定細(xi)胞(bao)活(huo)力。

Countess® II FL全自動細胞計數儀開箱

• 靈活—可針對您的應用選擇熒光濾光片
• *—可選配可重復使用的計數玻片，有助于節省耗材成本
• 精確—可自動對焦，提升了精確度，減少了差異性
• 簡單—采用易于使用的觸摸屏界面
   

全光譜熒光檢測

Countess® II FL全自(zi)動細胞(bao)計數(shu)儀(yi)具有兩路熒光(guang)(guang)(guang)通道。其(qi)所采集的熒光(guang)(guang)(guang)色通過插入單獨(du)的光(guang)(guang)(guang)立(li)方進行(xing)測(ce)(ce)定(ding)。Countess® II FL全自(zi)動細胞(bao)計數(shu)儀(yi)提(ti)供有超(chao)過15種(zhong)光(guang)(guang)(guang)立(li)方可供選擇，檢(jian)測(ce)(ce)不受表達GFP和(he)(he)RFP的細胞(bao)的限制。對于轉染了RFP表達載體，和(he)(he)藍(lan)色核復染的細胞(bao)，，您是否希(xi)望(wang)測(ce)(ce)定(ding)其(qi)轉染效(xiao)率(lv)？沒問題！您只需加入和(he)(he)RFP和(he)(he)DAPI光(guang)(guang)(guang)立(li)方，即可對整個細胞(bao)群之中表達RFP融合的細胞(bao)進行(xing)高效(xiao)地計數(shu)，同時還可精確地測(ce)(ce)定(ding)轉染效(xiao)率(lv)。

通過兩種顏色熒光進行細胞計數。將10 μL采用CellLight® Histone 2B-RFP轉導并采用NucBlue® Live ReadyProbes® 試劑染色的Hela細胞加入到腔室載玻片上，然后插入到配備了DAPI和RFP光立方的Countess® II FL全自動細胞計數儀上。新Countess® II FL全自動細胞計數儀的分割和計數算法可以根據細胞的熒光性質對其進行計數。在本實驗中，細胞群之中86%的Hela細胞被

Countess® II FL全自動細胞計數儀所采用的EVOS®光立方。

Countess® II FL全自動細(xi)胞計(ji)數(shu)儀的熒光(guang)(guang)檢(jian)測技術的核心是的EVOS® LED光(guang)(guang)立方。每個光(guang)(guang)立方含有(you)一個LED、發光(guang)(guang)光(guang)(guang)學(xue)器件和濾光(guang)(guang)片(pian)。光(guang)(guang)立方具有(you)插(cha)拔功能，可由用戶(hu)更換，并通過Countess®系統進(jin)行自動配(pei)置。這些類(lei)型(xing)廣(guang)泛的光(guang)(guang)立方為(wei)眾(zhong)多熒光(guang)(guang)研究應用（如細(xi)胞活(huo)力分析(xi)）提供了更大(da)的靈活(huo)性。

See the entire line of compatible EVOS® light cubes

The Countess® II FL Automated Cell Counter is a benchtop instrument designed and manufactured by Invitrogen, which performs cell counting and viability measurements in concert with trypan blue stain.  The Countess™ provides fast, easy and accurate cell counting without using a hemocytometer, thus eliminating the tedium and subjectivity of manual cell counting.
 
GIBCO® brand TrypLE™ Select and TrypLE™ Express are recombinant serine protease enzymes of high purity, which contain no components of animal origin.  TrypLE is designed to remove adherent cells from the surface of plastic flasks and plates in a gentler manner.  In addition, it is room temperature stable and a direct replacement for trypsin.

• This protocol details the procedure for obtaining accurate cell counts using TrypLE cell dissociation products with the Countess® II FL Automated Cell Counter.