Chromotek/Nuclear Actin-Chromobody

螞蟻淘常見平行(xing)進口產(chan)品有：

Thermofisher旗下Gibco培養系列、ABI細胞(bao)計(ji)數儀及PCR儀、Invitrogen分子生物學試劑及Qubit3.0、Ambion的RNA相關試劑、Molecular probes試劑、Pierce蛋白相(xiang)關試劑盒(he)、Nanodrop One/OneC；Parafilm封(feng)口膜；Viskase透析(xi)袋；Sigma-Aldrich試劑；AccuStandard 標準(zhun)品；Millipore抗(kang)體(ti)；Glen Research 核酸合(he)成試劑(ji)；Bio-Rad電泳儀及(ji)PCR儀；Nanocs的PEG聚合物；  Chromotek羊駝抗體；   Polysciences轉染(ran)試劑；  Echelon-inc 脂類研究(jiu)試劑盒；   R&D Systems抗體及(ji)Elisa試(shi)劑盒； Jackson二抗及封閉(bi)血清； Biotium 熒光染料；NEB內切酶及NGS相關產品；  Athens Research & Technology人類蛋白(bai)及抗血清；  Illumina二代測試試劑(ji)盒； Tocris 小分子試(shi)劑； TwistDx恒(heng)溫擴增(zeng)試劑盒(he)（RPA）； Enzymatics二(er)代測序酶；Novus抗體及Elisa試(shi)劑盒；AG scientific潮霉(mei)素B、G-418；Plantmedia植物培養系列及(ji)抗體；Agilent 2100生物(wu)分析儀(yi)；Biolog耗材；Biomatik的Elisa試劑盒；Covaris基因打(da)斷儀；CST抗體；Dharmacon的(de)siRNA產品。

Chromotek/Nuclear Actin-Chromobody

　　Visualize the actin dynamics in real time in the nucleus with the nuclear Actin-Chromobody®. Upon transformation, your cells express the nuclear Actin-Chromobody® and transport the nuclear Actin-Chromobody into the nucleus. The nuclear cytoskeleton is visualized in real time by binding of the fluorescent Chromobody® to Actin.Actin-Chromobody®

　　Courtesy of Matthias PlessnerNIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody® (TagGFP2, green) and the Lamin-Chromobody® (TagRFP2, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody-probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum.

　　Trace actin dynamics in cell nuclei non-invasively

　　With the nuclear Actin-Chromobody® plasmid you obtain the sequence of the Alpaca antibody to Actin, also termed VHH-Actin or Actin-nanobody, fused to TagGFP (Evrogen) and a nuclear localization sequence (NLS). The transient binding does not influence cell viability or motility and thus offers you the unique possibility to observe the cytoskeleton in your cell nuclei.

　　The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:

　　Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (PubMed)